opera phenix plus high-content screening system (Revvity)
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Revvity
opera phenix plus high-content screening system
Opera Phenix Plus High Content Screening System, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 2557 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/opera+phenix+high+content+screening+system/Opera+Phenix+Plus+high-content+screening+system/custom%40hh14001000%4042443179
Average 96 stars, based on 2557 article reviews
Opera Phenix Plus High Content Screening System, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 2557 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/opera+phenix+high+content+screening+system/Opera+Phenix+Plus+high-content+screening+system/custom%40hh14001000%4042443179
Average 96 stars, based on 2557 article reviews
opera phenix plus high-content screening system - by Bioz Stars,
2026-09
96/100 stars
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Staining:Article Title: Loss of schizophrenia risk gene XPO7 disrupts neuronal excitability and network regularity via altered Na + channel dynamics in human neurons. Article Snippet: .. Stained cells were imaged using the Article Title: Cell Painting PLUS: An Iterative Staining‐Elution Protocol for High‐Content Phenotypic Screenings Article Snippet: .. Cell assay plates (Basic Protocol or Alternate Protocol) 2× live‐cell staining solution (see recipe) 2× fixation solution (see recipe) Phosphate‐buffered saline (PBS; Santa Cruz, cat. no. sc‐362299) 2× cycle 1 staining and permeabilization solution (see recipe) CPP elution buffer (see recipe) 2× cycle 2 staining solution (see recipe) Automated liquid‐ and plate‐handling workstation: We use JANUS pipetting robot (Revvity Inc.), plate incubator (Liconic STX44 ICSA), microplate washer (BioTek 405LSUVS), and plate handler robot (Denso VS050A1‐AV6‐NNN) Appropriate pipetting tips and liquid reservoirs for the respective automated pipetting device: We use sterile 384‐well, 30‐μL Janus MDT filter tips (Revvity, cat. no. 6001620) and 384 pyramid base geometry reservoirs for Janus G3 (Agilent, cat. no. 204612‐100) High‐content imaging system equipped with 20× water objective and respective lasers and emission filter sets (Table ): We use an Article Title: SARS-CoV-2 variant-specific enhancement of dendritic heparan sulfate mimetic antiviral activities. Article Snippet: SARS-CoV-2 continues to impose a substantial burden to healthcare systems, economies, and communities.. While several antivirals are approved for COVID-19 treatment, their use is limited by contraindications and uptake, and vaccines provide only partial and waning protection against infection.. Therefore, additional antivirals are urgently needed to combat SARS-CoV-2. High Content Screening:Article Title: Loss of schizophrenia risk gene XPO7 disrupts neuronal excitability and network regularity via altered Na + channel dynamics in human neurons. Article Snippet: .. Stained cells were imaged using the Article Title: Loss of protein phosphatase 1 regulator TIMAP protein triggers EMT in A549 cells. Article Snippet: .. Imaging was performed with the Article Title: Glioblastoma invasion into different organoid hosts reveals cell-intrinsic and proliferative migratory programs Article Snippet: .. Time-lapse live-cell imaging was performed on the Article Title: Interactive renal toxicity of perfluorooctanoic acid and ethanol: Impairment of epithelial transport and redox homeostasis in male Sprague-Dawley rats. Article Snippet: .. Images were acquired using the Article Title: Cell Painting PLUS: An Iterative Staining‐Elution Protocol for High‐Content Phenotypic Screenings Article Snippet: .. Cell assay plates (Basic Protocol or Alternate Protocol) 2× live‐cell staining solution (see recipe) 2× fixation solution (see recipe) Phosphate‐buffered saline (PBS; Santa Cruz, cat. no. sc‐362299) 2× cycle 1 staining and permeabilization solution (see recipe) CPP elution buffer (see recipe) 2× cycle 2 staining solution (see recipe) Automated liquid‐ and plate‐handling workstation: We use JANUS pipetting robot (Revvity Inc.), plate incubator (Liconic STX44 ICSA), microplate washer (BioTek 405LSUVS), and plate handler robot (Denso VS050A1‐AV6‐NNN) Appropriate pipetting tips and liquid reservoirs for the respective automated pipetting device: We use sterile 384‐well, 30‐μL Janus MDT filter tips (Revvity, cat. no. 6001620) and 384 pyramid base geometry reservoirs for Janus G3 (Agilent, cat. no. 204612‐100) High‐content imaging system equipped with 20× water objective and respective lasers and emission filter sets (Table ): We use an Article Title: SARS-CoV-2 variant-specific enhancement of dendritic heparan sulfate mimetic antiviral activities. Article Snippet: SARS-CoV-2 continues to impose a substantial burden to healthcare systems, economies, and communities.. While several antivirals are approved for COVID-19 treatment, their use is limited by contraindications and uptake, and vaccines provide only partial and waning protection against infection.. Therefore, additional antivirals are urgently needed to combat SARS-CoV-2. Article Title: Monitoring pH-induced lipid dynamics in mRNA-lipid nanoparticles by synchrotron small-angle X-ray scattering. Article Snippet: Lipid nanoparticles (LNPs), a promising drug delivery system, face key limitations in terms of a poor understanding toward their assembling mechanism and intracellular dynamics.. Conventional characterization methods like dynamic light scattering and spectroscopy fail to capture the dynamic mesoscopic structural changes of LNPs under pH gradient.. To address this issue, here we employed synchrotron small-angle X-ray scattering (SR-SAXS) to ex-situ monitor the dynamic self-assembly process of mRNA-encapsulated LNPs at different physiologicallyrelevant pH. Imaging:Article Title: Loss of protein phosphatase 1 regulator TIMAP protein triggers EMT in A549 cells. Article Snippet: .. Imaging was performed with the Article Title: Cell Painting PLUS: An Iterative Staining‐Elution Protocol for High‐Content Phenotypic Screenings Article Snippet: .. Cell assay plates (Basic Protocol or Alternate Protocol) 2× live‐cell staining solution (see recipe) 2× fixation solution (see recipe) Phosphate‐buffered saline (PBS; Santa Cruz, cat. no. sc‐362299) 2× cycle 1 staining and permeabilization solution (see recipe) CPP elution buffer (see recipe) 2× cycle 2 staining solution (see recipe) Automated liquid‐ and plate‐handling workstation: We use JANUS pipetting robot (Revvity Inc.), plate incubator (Liconic STX44 ICSA), microplate washer (BioTek 405LSUVS), and plate handler robot (Denso VS050A1‐AV6‐NNN) Appropriate pipetting tips and liquid reservoirs for the respective automated pipetting device: We use sterile 384‐well, 30‐μL Janus MDT filter tips (Revvity, cat. no. 6001620) and 384 pyramid base geometry reservoirs for Janus G3 (Agilent, cat. no. 204612‐100) High‐content imaging system equipped with 20× water objective and respective lasers and emission filter sets (Table ): We use an Live Cell Imaging:Article Title: Glioblastoma invasion into different organoid hosts reveals cell-intrinsic and proliferative migratory programs Article Snippet: .. Time-lapse live-cell imaging was performed on the other:Article Title: Deep single-cell decoding of human pancreatic islets reveals T2D β-cell gene expression defects. Article Snippet: The lysate was centrifuged at 3000 rpm for 5 min and stored at -20 °C until human insulin ELISA (Mercodia) according to the manufacturer’s instructions. Saline:Article Title: Cell Painting PLUS: An Iterative Staining‐Elution Protocol for High‐Content Phenotypic Screenings Article Snippet: .. Cell assay plates (Basic Protocol or Alternate Protocol) 2× live‐cell staining solution (see recipe) 2× fixation solution (see recipe) Phosphate‐buffered saline (PBS; Santa Cruz, cat. no. sc‐362299) 2× cycle 1 staining and permeabilization solution (see recipe) CPP elution buffer (see recipe) 2× cycle 2 staining solution (see recipe) Automated liquid‐ and plate‐handling workstation: We use JANUS pipetting robot (Revvity Inc.), plate incubator (Liconic STX44 ICSA), microplate washer (BioTek 405LSUVS), and plate handler robot (Denso VS050A1‐AV6‐NNN) Appropriate pipetting tips and liquid reservoirs for the respective automated pipetting device: We use sterile 384‐well, 30‐μL Janus MDT filter tips (Revvity, cat. no. 6001620) and 384 pyramid base geometry reservoirs for Janus G3 (Agilent, cat. no. 204612‐100) High‐content imaging system equipped with 20× water objective and respective lasers and emission filter sets (Table ): We use an Sterility:Article Title: Cell Painting PLUS: An Iterative Staining‐Elution Protocol for High‐Content Phenotypic Screenings Article Snippet: .. Cell assay plates (Basic Protocol or Alternate Protocol) 2× live‐cell staining solution (see recipe) 2× fixation solution (see recipe) Phosphate‐buffered saline (PBS; Santa Cruz, cat. no. sc‐362299) 2× cycle 1 staining and permeabilization solution (see recipe) CPP elution buffer (see recipe) 2× cycle 2 staining solution (see recipe) Automated liquid‐ and plate‐handling workstation: We use JANUS pipetting robot (Revvity Inc.), plate incubator (Liconic STX44 ICSA), microplate washer (BioTek 405LSUVS), and plate handler robot (Denso VS050A1‐AV6‐NNN) Appropriate pipetting tips and liquid reservoirs for the respective automated pipetting device: We use sterile 384‐well, 30‐μL Janus MDT filter tips (Revvity, cat. no. 6001620) and 384 pyramid base geometry reservoirs for Janus G3 (Agilent, cat. no. 204612‐100) High‐content imaging system equipped with 20× water objective and respective lasers and emission filter sets (Table ): We use an Software:Article Title: Cell Painting PLUS: An Iterative Staining‐Elution Protocol for High‐Content Phenotypic Screenings Article Snippet: .. Cell assay plates (Basic Protocol or Alternate Protocol) 2× live‐cell staining solution (see recipe) 2× fixation solution (see recipe) Phosphate‐buffered saline (PBS; Santa Cruz, cat. no. sc‐362299) 2× cycle 1 staining and permeabilization solution (see recipe) CPP elution buffer (see recipe) 2× cycle 2 staining solution (see recipe) Automated liquid‐ and plate‐handling workstation: We use JANUS pipetting robot (Revvity Inc.), plate incubator (Liconic STX44 ICSA), microplate washer (BioTek 405LSUVS), and plate handler robot (Denso VS050A1‐AV6‐NNN) Appropriate pipetting tips and liquid reservoirs for the respective automated pipetting device: We use sterile 384‐well, 30‐μL Janus MDT filter tips (Revvity, cat. no. 6001620) and 384 pyramid base geometry reservoirs for Janus G3 (Agilent, cat. no. 204612‐100) High‐content imaging system equipped with 20× water objective and respective lasers and emission filter sets (Table ): We use an Article Title: SARS-CoV-2 variant-specific enhancement of dendritic heparan sulfate mimetic antiviral activities. Article Snippet: SARS-CoV-2 continues to impose a substantial burden to healthcare systems, economies, and communities.. While several antivirals are approved for COVID-19 treatment, their use is limited by contraindications and uptake, and vaccines provide only partial and waning protection against infection.. Therefore, additional antivirals are urgently needed to combat SARS-CoV-2. Fluorescence:Article Title: Cell Painting PLUS: An Iterative Staining‐Elution Protocol for High‐Content Phenotypic Screenings Article Snippet: .. Cell assay plates (Basic Protocol or Alternate Protocol) 2× live‐cell staining solution (see recipe) 2× fixation solution (see recipe) Phosphate‐buffered saline (PBS; Santa Cruz, cat. no. sc‐362299) 2× cycle 1 staining and permeabilization solution (see recipe) CPP elution buffer (see recipe) 2× cycle 2 staining solution (see recipe) Automated liquid‐ and plate‐handling workstation: We use JANUS pipetting robot (Revvity Inc.), plate incubator (Liconic STX44 ICSA), microplate washer (BioTek 405LSUVS), and plate handler robot (Denso VS050A1‐AV6‐NNN) Appropriate pipetting tips and liquid reservoirs for the respective automated pipetting device: We use sterile 384‐well, 30‐μL Janus MDT filter tips (Revvity, cat. no. 6001620) and 384 pyramid base geometry reservoirs for Janus G3 (Agilent, cat. no. 204612‐100) High‐content imaging system equipped with 20× water objective and respective lasers and emission filter sets (Table ): We use an Article Title: Monitoring pH-induced lipid dynamics in mRNA-lipid nanoparticles by synchrotron small-angle X-ray scattering. Article Snippet: Lipid nanoparticles (LNPs), a promising drug delivery system, face key limitations in terms of a poor understanding toward their assembling mechanism and intracellular dynamics.. Conventional characterization methods like dynamic light scattering and spectroscopy fail to capture the dynamic mesoscopic structural changes of LNPs under pH gradient.. To address this issue, here we employed synchrotron small-angle X-ray scattering (SR-SAXS) to ex-situ monitor the dynamic self-assembly process of mRNA-encapsulated LNPs at different physiologicallyrelevant pH. Incubation:Article Title: Monitoring pH-induced lipid dynamics in mRNA-lipid nanoparticles by synchrotron small-angle X-ray scattering. Article Snippet: Lipid nanoparticles (LNPs), a promising drug delivery system, face key limitations in terms of a poor understanding toward their assembling mechanism and intracellular dynamics.. Conventional characterization methods like dynamic light scattering and spectroscopy fail to capture the dynamic mesoscopic structural changes of LNPs under pH gradient.. To address this issue, here we employed synchrotron small-angle X-ray scattering (SR-SAXS) to ex-situ monitor the dynamic self-assembly process of mRNA-encapsulated LNPs at different physiologicallyrelevant pH. |